samir123
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Apr 06, 2025
9:39 PM
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HBTU ( Hexafluorophosphate Benzotriazole Tetramethyl Uronium ) is a widely used coupling reagent in peptide synthesis and organic chemistry. Due to its efficiency, low racemization, and versatility, it has become an essential tool for researchers and pharmaceutical industries. This blog will explore the benefits, uses, and applications of HBTU in various chemical fields.
What is HBTU?
HBTU is an amide bond-forming reagent primarily used in solid-phase peptide synthesis (SPPS) and solution-phase synthesis. It enables the efficient coupling of amino acids and carboxylic acids to form peptides, proteins, and other bioactive molecules.
Chemical Information
Chemical Name: Hexafluorophosphate Benzotriazole Tetramethyl Uronium
Molecular Formula: C11H16F6N5OP
Molecular Weight: 379.25 g/mol
CAS Number: 94790-37-1
Appearance: White to off-white crystalline powder
7 Powerful Benefits & Uses of HBTU
1. High Efficiency in Peptide Synthesis
HBTU is one of the most effective reagents for forming amide bonds, making it indispensable in peptide chemistry. It:
Works rapidly under mild conditions
Provides high yield and selectivity
Minimizes unwanted by-productsHBTU (Hexafluorophosphate Benzotriazole Tetramethyl Uronium) is a widely used coupling reagent in peptide synthesis and organic chemistry. Due to its efficiency, low racemization, and versatility, it has become an essential tool for researchers and pharmaceutical industries. This blog will explore the benefits, uses, and applications of HBTU in various chemical fields.
What is HBTU?
HBTU is an amide bond-forming reagent primarily used in solid-phase peptide synthesis (SPPS) and solution-phase synthesis. It enables the efficient coupling of amino acids and carboxylic acids to form peptides, proteins, and other bioactive molecules.
Chemical Information
Chemical Name: Hexafluorophosphate Benzotriazole Tetramethyl Uronium
Molecular Formula: C11H16F6N5OP
Molecular Weight: 379.25 g/mol
CAS Number: 94790-37-1
Appearance: White to off-white crystalline powder
2. Low Racemization for Stereochemical Purity
One major challenge in peptide synthesis is epimerization, which can lead to undesired stereoisomers. HBTU significantly reduces racemization, ensuring that the final product retains the correct stereochemistry.
3. Versatility in Organic Synthesis
Beyond peptide synthesis, HBTU is used to create:
Amides (Carboxyl to Amide Conversion)
Ureas and Carbamates
Peptidomimetics (Mimicking peptide structures in drug discovery)
4. Ideal for Solid-Phase and Solution-Phase Synthesis
HBTU is compatible with both solid-phase peptide synthesis (SPPS) and solution-phase synthesis, allowing for:
Efficient fragment condensation
Easier purification steps
Compatibility with various solvents like DMF, DMSO, and CH?Cl?
5. Advantage Over Traditional Coupling Reagents
Compared to older reagents like DCC, DIC, and EDC, HBTU provides:
Faster reaction rates
Higher efficiency
Easier handling (non-toxic and more soluble by-products)
6. Essential in Pharmaceutical Research
HBTU is widely used in drug discovery and biomedical research, including:
Synthesis of antibiotics and antiviral drugs
Production of peptide-based vaccines
Development of cancer therapeutics
7. Safe and Easy to Handle
HBTU is stable under normal laboratory conditions and does not require special storage. It dissolves well in most organic solvents, making it convenient for both small-scale and industrial applications.
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